Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Luminescence applications and assays. Recertification service. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. M5. 76358-624. 057, depending on lot number. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Microplate optimization and read height optimization were performed for best results. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. d. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. Optional 16-way manifold. Next-generation luminescence plate reader. When a. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Integrated stacker option. UV absorbance detection. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. The IC50s by cell rounding and LysoTracker. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. 0% ± 0. Microplate Spectrophotometers. 1 fmol luciferase per well. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. $3,450. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Published Dec 16, 2020 | Updated Dec 16, 2020. Values are averages from 5 clean plates, each filled with 200 µL water/well. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The equation that allows one to calculate absorbance from % transmittance is. Cell viability and cytoxicity assays. CO 2 & O 2 control. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. T = I/I 0 and %T = 100 (T). 2. Figure 3. 00 . the use of a reader calibrated with primary NIST and NMI standards. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. 6-384 (96- and 384-well with pipetting) 96-well microplates. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. 0 . Four wash/rinse bottles are included and configurable for both 96- and 384. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. 304 signal level saturation. x. 00. Published Dec 16,. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. (one set per assay) to clear 96-well microplates. Supersedes 1. Infinite possibilities. 2. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Time-resolved phosphorescence. Uncontrolled Copy for Training and Reference Purposes Only . : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. . The procedures in this protocol support the 96-well reaction plate. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 96-, 384-, and 1536-well microplates. 5 ng per well. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. A custom 96-well plastic (polyethylene terephthalate/PET) plate is attached via epoxy to the mounted CMOS ICs, designed with a standard form factor to be compatible with high-throughput. Versa Max. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 96-well plates - Filter plates with 0. Anticancer drug assay was also conducted using conventional 96-well plates as controls. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Z. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. High tolerance for contaminants. 96-, 384-, and 1536-well microplates. 1 mL/well of media. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Click on the crosshair and move it so that it is centered over the well image. This page contains the. Conventional technique: subjective, time-consuming. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. 000 – 4. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. #C1500) Control standard endotoxin (ACC cat. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. External Dimensions without lid (mm). In SoftMax Pro, Dual Read mode was selected. Features. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. No. 0 . To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Product Compare 0. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. x. Gemini EM Reader scans. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. Fluorescence Microplate Reader Comparison Chart. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. It features a modular design that allows users to add or change detection cartridges according to their needs. Fluorescence Microplate Reader Comparison Chart. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Molecular Devices SpectraMax Plus 384 Absorbance. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. 25% and 0. The color touch screen provides a visual user interface, making programming fast and intuitive. This reader's dual monochromators for. Part Number. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). 020 fmol 0. (Molecular Devices cat. Bay Gray powder coated metal back plate, column, and base. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. The system features 96, 384,. Important things to consider when purchasing a multimode microplate reader. QC tools and IQOQ services. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 012 fmol 0. 000 to 2. 300 µL of Detergent Compatible Bradford reagent was added to each well. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. Populate the following info under Description. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. 1% in white 96- and < 0. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Self-righting base, up to 40 degrees past center without dispenser. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Make sure toPlate reader instructions Computer. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. 1 x plates; Instrument dimensions. In addition, the iD5 reader measures TRF and FP and. Growth medium was then replaced with fatty acid uptake solution. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. 96-, 384-, and 1536-well microplates. 0 OD. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. 2. Supplier: Molecular Devices. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Absorbance cuvette port. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. Figure 3. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. Compare this item. • Easy and gentle immobilization of biotin. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Access this by going to. B. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Quantitative, whole well cell confluence for every well. These lab workhorses are used in drug discovery. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. . These microplates have a corner notch for use in standard plate readers. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. The soil was amended with either 1. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 306 plate air cal. Molecular Devices has provided scientists with . SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. • Remove media from each well using a blue pipette tip being careful as not to remove cells. Place the microplate in the drawer. The drawing shows a standard microplate of 15 mm height covered with a lid. Source plate capacity; 1 x plates. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 2. White plates reflect the light, enabling even low levels of Luminescence to be detected. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Features. A = Log 10 (I 0 /I). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. com. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. variety of. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Automated live cell imaging – cell counting and confluence. 2 with 1000 cells. $3,207. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. 000 OD ± 1. * Note: Programming the washer with a final aspirate height of 4. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). #23225). Or scan up to 384 wells at any time to determine the best assay conditions. Important things to consider when purchasing a multimode microplate reader. #3693; n = 4 for each standard). 340 PC384. UV absorbance detection. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. Your applications, your modes, your choice. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. • To avoid removing loosely attached cells don’t wash if the background signal is not too highTeams measured absorbance at 600 nm (OD600) and GFP in a plate reader from four technical replicates per biological replicate (for a total of eight replicates and fitting on a 96-well plate) at. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Bioz Stars score: 86/100, based on 40 PubMed citations. Polypropylene deep well plates, round bottom. 313 reference gain check fail. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. 039. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . 00 USD. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . Sample by eye within . to each well. Return to top. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 5L) MultiWash+. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 96 well plate injection time with SpectraMax® L plate reader. 100 fmol 384-well (75 µL) 0. x. The plate reader optics are tunable for. Please refer to the Obsolete Microplate Reader Models for more information. x. the Gemini XPS reader. Click SoftMax Pro Software User Guide. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Molecular Devices L-Max II Luminescence Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Specifications subject to change without notice. This can increase throughput and efficiency, thus saving time and space in the lab. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. $488. Fluorescence Microplate Reader Comparison Chart. 000 to 2. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Microplates, cuvettes (via adapter) Detectors. Plate and Setup Rack Options Screens with selection of up to four Methods shown. REF (SpectraMax. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. adjust the reader. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Service parts for these models are not being produced and are no longer in stock. Learn more. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 00. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. . 150 µL of deionized water was pipetted into each well of a clean 96-well plate. 984. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. 1. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. 340 PC384. (Molecular Devices cat. Please click on the links below to view the Microplate Reader Comparison Tables. minutes and begin reading microplates with seven mouse clicks. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. 003 fmol 0. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 9000-0756. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. Figure 1. Plate. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Other technologies such as imaging. Published Dec 16, 2020 | Updated Dec 16, 2020. 9. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. Incubation. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Learn how you can utilize microplate readers to drive landmark discoveries. 7°C (gradient heating design). For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. Selects either 96-well or 384-well plate for a stand-alone read. 303 unable to cal dark current. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. No accessories are needed and SoftMax® Pro Software. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 20 attomol ATP per well. x. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 0, or. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. May require LS-50B…. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. 312 gain calibration failed. Place the microplate in the drawer. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Artel MVS Bundle DM Std/800TSNB Reader Details. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Intuitive graphical user interface makes each assay extremely easy to set. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. Align the two slots on the bottle holder with the two pegs on the rail. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates.